产品描述
This Human EDN1 ELISA Kit was designed for the quantitative measurement of Human EDN1 protein in serum, cell culture supernates, urine, saliva. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
ARCND3 ELISA Kit; Big endothelin-1 ELISA Kit; EDN1 ELISA Kit; EDN1_HUMAN ELISA Kit; ET-1 ELISA Kit; ET1 ELISA Kit; HDLCQ7 ELISA Kit; PPET1 ELISA Kit; Preproendothelin 1 ELISA Kit; Preproendothelin-1 ELISA Kit; QME ELISA Kit
缩写
EDN1
Uniprot No
P05305
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 82-97 | |
| 1:2 | Average % | 98 | |
| Range % | 94-103 | |
| 1:4 | Average % | 97 | |
| Range % | 90-105 | |
| 1:8 | Average % | 87 | |
| Range % | 82-91 | |
回收率
| The recovery of human ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.722 | 2.752 | 2.737 | 2.628 | | | 100 | 2.169 | 2.322 | 2.246 | 2.137 | | | 50 | 1.545 | 1.528 | 1.537 | 1.428 | | | 25 | 0.882 | 0.901 | 0.892 | 0.783 | | | 12.5 | 0.551 | 0.575 | 0.563 | 0.454 | | | 6.25 | 0.341 | 0.351 | 0.346 | 0.237 | | | 3.12 | 0.245 | 0.245 | 0.245 | 0.136 | | | 0 | 0.112 | 0.105 | 0.109 | | |
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货期
3-5 working days