产品描述
This Human GDF9 ELISA Kit was designed for the quantitative measurement of Human GDF9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 43 pg/mL-1500 pg/mL and the sensitivity is 22 pg/mL.
别名
GDF9Growth/differentiation factor 9 ELISA Kit; GDF-9 ELISA Kit
缩写
GDF9
Uniprot No
O60383
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GDF-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-106 |
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| 1:2 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:4 |
Average % |
103 |
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| Range % |
99-107 |
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| 1:8 |
Average % |
97 |
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| Range % |
91-102 |
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回收率
| The recovery of human GDF-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
89 |
84-92 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1500 |
1.683 |
1.744 |
1.714 |
1.666 |
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| 535 |
0.791 |
0.775 |
0.783 |
0.735 |
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| 214 |
0.388 |
0.392 |
0.390 |
0.342 |
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| 107 |
0.237 |
0.246 |
0.242 |
0.194 |
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| 42.8 |
0.121 |
0.126 |
0.124 |
0.076 |
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| 0 |
0.047 |
0.048 |
0.048 |
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货期
3-5 working days