产品描述
This Mouse GPC3 ELISA Kit was designed for the quantitative measurement of Mouse GPC3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
别名
Gpc3 ELISA Kit; Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit
缩写
GPC3
Uniprot No
Q8CFZ4
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPC-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 92 | | |
| Range % | 84-95 | | |
| 1:2 | Average % | 94 | | |
| Range % | 84-98 | | |
| 1:4 | Average % | 94 | | |
| Range % | 85-97 | | |
| 1:8 | Average % | 90 | | |
| Range % | 84-94 | | |
回收率
| The recovery of mouse GPC-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 99 | 91-103 | | |
| EDTA plasma (n=4) | 95 | 90-99 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 200 | 2.173 | 2.202 | 2.188 | 2.050 | | | | 100 | 1.648 | 1.683 | 1.666 | 1.528 | | | | 50 | 0.936 | 0.911 | 0.924 | 0.786 | | | | 25 | 0.549 | 0.540 | 0.545 | 0.407 | | | | 12.5 | 0.301 | 0.323 | 0.312 | 0.174 | | | | 6.25 | 0.245 | 0.251 | 0.248 | 0.110 | | | | 3.12 | 0.178 | 0.181 | 0.180 | 0.042 | | | | 0 | 0.137 | 0.139 | 0.138 | | | |
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货期
3-5 working days