产品描述
This Rat CXCL1 ELISA Kit was designed for the quantitative measurement of Rat CXCL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.
别名
Cxcl1 ELISA Kit; Cinc1 ELISA Kit; Gro ELISA Kit; Scyb1Growth-regulated alpha protein ELISA Kit; C-X-C motif chemokine 1 ELISA Kit; Cytokine-induced neutrophil chemoattractant 1 ELISA Kit; CINC-1 ELISA Kit; Platelet-derived growth factor-inducible protein KC ELISA Kit
缩写
CXCL1
Uniprot No
P14095
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GROα /MGSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 91 | | |
| Range % | 86-95 | | |
| 1:2 | Average % | 106 | | |
| Range % | 101-112 | | |
| 1:4 | Average % | 93 | | |
| Range % | 87-96 | | |
| 1:8 | Average % | 93 | | |
| Range % | 86-98 | | |
回收率
| The recovery of rat GROα /MGSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 94 | 89-98 | | |
| EDTA plasma (n=4) | 99 | 96-104 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 8000 | 2.454 | 2.589 | 2.522 | 2.396 | | | | 4000 | 2.012 | 2.180 | 2.096 | 1.970 | | | | 2000 | 1.445 | 1.496 | 1.471 | 1.345 | | | | 1000 | 0.784 | 0.809 | 0.797 | 0.671 | | | | 500 | 0.433 | 0.441 | 0.437 | 0.311 | | | | 250 | 0.236 | 0.249 | 0.243 | 0.117 | | | | 125 | 0.173 | 0.179 | 0.176 | 0.050 | | | | 0 | 0.125 | 0.127 | 0.126 | | | |
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货期
3-5 working days