产品描述
This Rat G-CSF ELISA Kit was designed for the quantitative measurement of Rat G-CSF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .
别名
Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
缩写
G-CSF
Uniprot No
P48750
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 86-94 | |
| 1:2 | Average % | 101 | |
| Range % | 97-104 | |
| 1:4 | Average % | 95 | |
| Range % | 90-97 | |
| 1:8 | Average % | 100 | |
| Range % | 95-103 | |
回收率
| The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-96 | |
| EDTA plasma (n=4) | 94 | 92-96 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.133 | 2.118 | 2.126 | 2.007 | | | 1000 | 1.837 | 1.776 | 1.807 | 1.688 | | | 500 | 1.294 | 1.282 | 1.288 | 1.169 | | | 250 | 0.889 | 0.878 | 0.884 | 0.765 | | | 125 | 0.588 | 0.607 | 0.598 | 0.479 | | | 62.5 | 0.361 | 0.349 | 0.355 | 0.236 | | | 31.25 | 0.222 | 0.216 | 0.219 | 0.100 | | | 0 | 0.118 | 0.120 | 0.119 | | |
|
货期
3-5 working days