产品描述
This Human HLA-E ELISA Kit was designed for the quantitative measurement of Human HLA-E protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
HLA-E ELISA Kit; HLA-6.2 ELISA Kit; HLAEHLA class I histocompatibility antigen ELISA Kit; alpha chain E ELISA Kit; MHC class I antigen E) [Cleaved into: Soluble HLA class I histocompatibility antigen ELISA Kit; alpha chain E ELISA Kit; sHLA-E)] ELISA Kit
缩写
HLA-E
Uniprot No
P13747
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
85-97 |
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| 1:2 |
Average % |
98 |
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| Range % |
90-102 |
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| 1:4 |
Average % |
95 |
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| Range % |
84-99 |
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| 1:8 |
Average % |
103 |
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| Range % |
95-107 |
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回收率
| The recovery of human HLA-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
88-104 |
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| EDTA plasma (n=4) |
97 |
88-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
1.976 |
1.943 |
1.960 |
1.788 |
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| 1000 |
1.499 |
1.446 |
1.473 |
1.301 |
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| 500 |
1.018 |
0.987 |
1.003 |
0.831 |
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| 250 |
0.741 |
0.712 |
0.727 |
0.555 |
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| 125 |
0.457 |
0.435 |
0.446 |
0.274 |
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| 62.5 |
0.335 |
0.323 |
0.329 |
0.157 |
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| 31.25 |
0.249 |
0.244 |
0.247 |
0.075 |
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| 0 |
0.175 |
0.169 |
0.172 |
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货期
3-5 working days