产品描述

This Human EPHA4 ELISA Kit was designed for the quantitative measurement of Human EPHA4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

Cek 8 ELISA Kit; CEK8 ELISA Kit; EK8 ELISA Kit; eph receptor a4 ELISA Kit; EPH-like kinase 8 ELISA Kit; EPHA4 ELISA Kit; EPHA4_HUMAN ELISA Kit; Ephrin type-A receptor 4 ELISA Kit; HEK 8 ELISA Kit; hEK8 ELISA Kit; Receptor protein-tyrosine kinase HEK8 ELISA Kit; Sek 1 ELISA Kit; SEK ELISA Kit; TYRO 1 protein tyrosine kinase ELISA Kit; TYRO1 ELISA Kit; Tyrosine-protein kinase receptor SEK ELISA Kit; Tyrosine-protein kinase TYRO1 ELISA Kit

缩写

EPHA4

Uniprot No

P54764

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human EPHA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		90-95
1:2		Average %		87
		Range %		85-89
1:4		Average %		87
		Range %		82-90
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human EPHA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		85-92
EDTA plasma (n=4)		87		85-90

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1600 2.328 2.368 2.348 2.214 800 1.551 1.598 1.575 1.441 400 1.004 1.038 1.021 0.887 200 0.562 0.575 0.569 0.435 100 0.397 0.381 0.389 0.255 50 0.265 0.278 0.272 0.138 25 0.201 0.209 0.205 0.071 0 0.135 0.132 0.134

货期

3-5 working days