产品描述
This Rat IGFBP1 ELISA Kit was designed for the quantitative measurement of Rat IGFBP1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit
缩写
IGFBP1
Uniprot No
P21743
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
87 |
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| Range % |
80-94 |
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| 1:200 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:400 |
Average % |
88 |
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| Range % |
81-95 |
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| 1:800 |
Average % |
100 |
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| Range % |
95-94 |
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回收率
| The recovery of rat IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-99 |
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| EDTA plasma (n=4) |
92 |
87-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.142 |
2.287 |
2.215 |
2.030 |
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| 500 |
1.882 |
1.897 |
1.890 |
1.705 |
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| 250 |
1.297 |
1.324 |
1.311 |
1.126 |
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| 125 |
0.803 |
0.815 |
0.809 |
0.624 |
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| 62.5 |
0.492 |
0.506 |
0.499 |
0.314 |
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| 31.2 |
0.360 |
0.387 |
0.374 |
0.189 |
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| 15.6 |
0.273 |
0.288 |
0.281 |
0.096 |
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| 0 |
0.184 |
0.186 |
0.185 |
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货期
3-5 working days