产品描述
This Rabbit ICAM-1/CD54 ELISA Kit was designed for the quantitative measurement of Rabbit ICAM-1/CD54 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .
缩写
ICAM-1/CD54
Uniprot No
U3KPG6
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 94 | |
| Range % | 91-98 | |
| 1:10 | Average % | 99 | |
| Range % | 91-106 | |
| 1:20 | Average % | 87 | |
| Range % | 82-94 | |
| 1:40 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of rabbit ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-96 | |
| EDTA plasma (n=4) | 100 | 96-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.694 | 2.594 | 2.644 | 2.501 | | | 20 | 2.144 | 2.044 | 2.094 | 1.951 | | | 10 | 1.324 | 1.284 | 1.304 | 1.161 | | | 5 | 0.754 | 0.734 | 0.744 | 0.601 | | | 2.5 | 0.536 | 0.546 | 0.541 | 0.398 | | | 1.25 | 0.351 | 0.361 | 0.356 | 0.213 | | | 0.625 | 0.220 | 0.230 | 0.225 | 0.082 | | | 0 | 0.142 | 0.143 | 0.143 | | |
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货期
3-5 working days