产品描述

This Human HSPA8 ELISA Kit was designed for the quantitative measurement of Human HSPA8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

2410008N15Rik ELISA Kit; Constitutive heat shock protein 70 ELISA Kit; Epididymis luminal protein 33 ELISA Kit; Epididymis secretory sperm binding protein Li 72p ELISA Kit; Heat shock 70 kDa protein 8 ELISA Kit; Heat shock 70kD protein 10 ELISA Kit; Heat shock 70kD protein 8 ELISA Kit; Heat shock 70kDa protein 8 ELISA Kit; Heat shock cognate 71 kDa protein ELISA Kit; Heat shock cognate protein 54 ELISA Kit; Heat shock cognate protein 71 kDa ELISA Kit; Heat shock protein 8 ELISA Kit; Heat shock protein A8 ELISA Kit; Heat shock protein family A (Hsp70) member 8 ELISA Kit; Heat-shock70-KD protein 10, formerly ELISA Kit; HEL 33 ELISA Kit; HEL S 72p ELISA Kit; HSC54 ELISA Kit; HSC71 ELISA Kit; Hsc73 ELISA Kit; HSP71 ELISA Kit; HSP73 ELISA Kit; HSP7C_HUMAN ELISA Kit; HSPA10 ELISA Kit; HSPA8 ELISA Kit; LAP1 ELISA Kit; Lipopolysaccharide associated protein 1 ELISA Kit; LPS associated protein 1 ELISA Kit; LPS associated protein ELISA Kit; MGC102007 ELISA Kit; MGC106514 ELISA Kit; MGC114311 ELISA Kit; MGC118485 ELISA Kit; MGC131511 ELISA Kit; MGC29929 ELISA Kit; N-myristoyltransferase inhibitor protein 71 ELISA Kit; NIP71 ELISA Kit

缩写

HSPA8

Uniprot No

P11142

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HSPA8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		89-99
1:2		Average %		88
		Range %		83-92
1:4		Average %		91
		Range %		84-95
1:8		Average %		100
		Range %		91-105

回收率

The recovery of human HSPA8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		82-94
EDTA plasma (n=4)		98		87-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     2000 1.868 1.913 1.891 1.719     1000 1.545 1.527 1.536 1.364     500 1.166 1.124 1.145 0.973     250 0.803 0.825 0.814 0.642     125 0.501 0.514 0.508 0.336     62.5 0.378 0.371 0.375 0.203     31.25 0.279 0.266 0.273 0.101     0 0.175 0.169 0.172

货期

3-5 working days