产品描述

This Human HSPD1 ELISA Kit was designed for the quantitative measurement of Human HSPD1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

别名

60 kDa chaperonin ELISA Kit; 60 kDa heat shock protein; mitochondrial ELISA Kit; CH60_HUMAN ELISA Kit; Chaperonin 60 ELISA Kit; Chaperonin; 60-KD ELISA Kit; CPN60 ELISA Kit; fa04a05 ELISA Kit; GROEL ELISA Kit; heat shock 60kDa protein 1 (chaperonin) ELISA Kit; Heat shock protein 1 (chaperonin) ELISA Kit; Heat shock protein 60 ELISA Kit; Heat shock protein 65 ELISA Kit; heat shock protein family D (Hsp60) member 1 ELISA Kit; HLD4 ELISA Kit; Hsp 60 ELISA Kit; HSP 65 ELISA Kit; HSP-60 ELISA Kit; HSP60 ELISA Kit; HSP65 ELISA Kit; HSPD1 ELISA Kit; HuCHA60 ELISA Kit; Mitochondrial matrix protein P1 ELISA Kit; P60 lymphocyte protein ELISA Kit; short heat shock protein 60 Hsp60s1 ELISA Kit; SPG13 ELISA Kit

缩写

HSPD1

Uniprot No

P10809

灵敏度

0.39 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-60 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		90-100
1:2		Average %		93
		Range %		88-99
1:4		Average %		87
		Range %		83-96
1:8		Average %		90
		Range %		86-84

回收率

The recovery of human HSP-60 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		87		82-90
EDTA plasma (n=4)		90		85-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 100 2.821 2.802 2.812 2.690 50 1.886 1.766 1.826 1.704 25 1.236 1.232 1.234 1.112 12.5 0.719 0.702 0.711 0.589 6.25 0.383 0.378 0.381 0.259 3.12 0.293 0.278 0.286 0.164 1.56 0.190 0.178 0.184 0.062 0 0.125 0.119 0.122

货期

3-5 working days