产品描述

The human HABP2 ELISA Kit is engineered for accurate measurement of human HABP2 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the human HABP2 content in the sample. The color intensity is positively correlated with HABP2 content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

HABP2, also called FSAP, is an extracellular serine protease involved in the extrinsic pathway of blood coagulation via activation of factor VII and fibrinolysis via activation of pro-urokinase type plasminogen activator (pro-uPA). It negatively regulates vascular integrity via activation of PAR receptor/RhoA/ROCK signaling. It has been implicated in several disease processes including atherosclerosis and deep venous thrombosis. Upregulation of HABP2 has been found in the pulmonary vasculature with acute lung injury (ALI) and promotes disruption of vascular integrity.

别名

Factor seven activating protease ELISA Kit; Factor seven-activating protease ELISA Kit; Factor VII activating protein ELISA Kit; Factor VII-activating protease ELISA Kit; FSAP ELISA Kit; HABP 2 ELISA Kit; HABP ELISA Kit; Habp2 ELISA Kit; HABP2_HUMAN ELISA Kit; Hepatocyte growth factor activator like protein ELISA Kit; Hepatocyte growth factor activator-like protein ELISA Kit; HGFAL ELISA Kit; Hyaluronan binding protein 2 ELISA Kit; Hyaluronan-binding protein 2 27 kDa light chain alternate form ELISA Kit; Hyaluronic acid binding protein 2 ELISA Kit; PHBP ELISA Kit; Plasma hyaluronan binding protein ELISA Kit; Plasma hyaluronan-binding protein ELISA Kit

缩写

HABP2

Uniprot No

Q14520

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HABP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:20		Average %		88
		Range %		82-96
1:40		Average %		89
		Range %		85-95
1:80		Average %		96
		Range %		91-101
1:160		Average %		100
		Range %		97-103

回收率

The recovery of human HABP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		99-105
EDTA plasma (n=4)		92		85-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.875 2.775 2.825 2.725 10 2.524 2.345 2.435 2.335 5 1.818 1.787 1.803 1.703 2.5 0.964 0.901 0.933 0.833 1.25 0.602 0.586 0.594 0.494 0.625 0.400 0.425 0.413 0.313 0.312 0.205 0.195 0.200 0.100 0 0.101 0.098 0.100

货期

3-5 working days