产品描述
This Sheep IL8 ELISA Kit was designed for the quantitative measurement of Sheep IL8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
别名
CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
缩写
CXCL8
Uniprot No
P36925
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 85 | | |
| Range % | 80-90 | | |
| 1:2 | Average % | 88 | | |
| Range % | 80-95 | | |
| 1:4 | Average % | 94 | | |
| Range % | 87-99 | | |
| 1:8 | Average % | 97 | | |
| Range % | 90-105 | | |
回收率
| The recovery of sheep IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 85-100 | | |
| EDTA plasma (n=4) | 96 | 91-103 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 400 | 2.312 | 2.231 | 2.272 | 2.094 | | | | 200 | 1.753 | 1.854 | 1.804 | 1.626 | | | | 100 | 1.289 | 1.339 | 1.314 | 1.136 | | | | 50 | 0.824 | 0.887 | 0.856 | 0.678 | | | | 25 | 0.558 | 0.597 | 0.578 | 0.400 | | | | 12.5 | 0.492 | 0.472 | 0.482 | 0.304 | | | | 6.25 | 0.308 | 0.297 | 0.303 | 0.125 | | | | 0 | 0.184 | 0.171 | 0.178 | | | |
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货期
3-5 working days