产品描述
CUSABIO's Hsp90 mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Hsp90 in serum, plasma, or tissue homogenates. This assay uses an antibody specific to mouse Hsp90 coated on a 96-well plate. Hsp90 present in a sample is bound to the wells by the immobilized antibody after adding the sample to the wells. Biotinylated Hsp90 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of Hsp90 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.
Hsp90 is a highly abundant and ubiquitous molecular chaperone essential for many cellular processes including cell cycle control, cell survival, hormone, and other signaling pathways. Hsp90 takes part in the proper folding and maintenance of its client proteins in eukaryotic cells including protein kinases, transcription factors, and E3 ubiquitin ligases, thus positioning as a central regulator of cellular proteostasis. It is also important for the cell's response to stress and is a key player in maintaining cellular homeostasis.
别名
Hsp90aa1 ELISA Kit; Hsp86 ELISA Kit; Hsp86-1 ELISA Kit; HspcaHeat shock protein HSP 90-alpha ELISA Kit; Heat shock 86 kDa ELISA Kit; HSP 86 ELISA Kit; HSP86 ELISA Kit; Tumor-specific transplantation 86 kDa antigen ELISA Kit; TSTA ELISA Kit
缩写
HSP90AA1
Uniprot No
P07901
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hsp-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 93 | |
| Range % | 81-101 | |
| 1:10 | Average % | 95 | |
| Range % | 86-104 | |
| 1:20 | Average % | 95 | |
| Range % | 82-105 | |
| 1:40 | Average % | 93 | |
| Range % | 82-101 | |
回收率
| The recovery of mouse Hsp-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-105 | |
| EDTA plasma (n=4) | 95 | 89-110 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.773 | 2.787 | 2.780 | 2.665 | | | 5 | 1.842 | 1.740 | 1.791 | 1.676 | | | 2.5 | 1.105 | 1.164 | 1.135 | 1.020 | | | 1.25 | 0.668 | 0.673 | 0.671 | 0.556 | | | 0.625 | 0.398 | 0.403 | 0.401 | 0.286 | | | 0.312 | 0.282 | 0.266 | 0.274 | 0.159 | | | 0.156 | 0.159 | 0.152 | 0.156 | 0.041 | | | 0 | 0.112 | 0.118 | 0.115 | | |
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货期
3-5 working days
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