产品描述
ICAM1是一种细胞间粘附分子,主要参与免疫细胞与其他细胞间的黏附,是炎症、感染、肿瘤等疾病的重要生物标志物。ICAM1的表达与白血病、肿瘤、心血管疾病等疾病密切相关,检测ICAM1水平可用于疾病诊断、治疗和监测。
华美生物所提供的Mouse intercellular adhesion molecule 1,ICAM-1 ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的ICAM1,其灵敏度为19.5 ng/mL,检测范围为78 ng/mL-5000 ng/mL。
别名
Icam1; Icam-1; Intercellular adhesion molecule 1; ICAM-1; MALA-2; MyD10; CD antigen CD54
缩写
ICAM1
Uniprot No
P13597
灵敏度
19.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
97 |
|
| Range % |
91-105 |
|
| 1:2 |
Average % |
85 |
|
| Range % |
80-92 |
|
| 1:4 |
Average % |
96 |
|
| Range % |
85-105 |
|
| 1:8 |
Average % |
93 |
|
| Range % |
83-98 |
|
回收率
| The recovery of mouse ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
82-101 |
|
| EDTA plasma (n=4) |
94 |
86-99 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 5000 |
2.555 |
2.518 |
2.537 |
2.390 |
|
| 2500 |
2.047 |
2.105 |
2.076 |
1.929 |
|
| 1250 |
1.531 |
1.552 |
1.542 |
1.395 |
|
| 625 |
0.812 |
0.803 |
0.808 |
0.661 |
|
| 312 |
0.516 |
0.524 |
0.520 |
0.373 |
|
| 156 |
0.312 |
0.321 |
0.317 |
0.170 |
|
| 78 |
0.229 |
0.225 |
0.227 |
0.080 |
|
| 0 |
0.145 |
0.149 |
0.147 |
|
|
|
货期
3-5 working days