产品描述

This Human HSP90AA1 ELISA Kit was designed for the quantitative measurement of Human HSP90AA1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .

别名

Heat shock 86 kDa ELISA Kit; Heat shock protein 90kDa alpha cytosolic class A member 1 ELISA Kit; Heat shock protein 90kDa alpha cytosolic class B member 1 ELISA Kit; Heat shock protein HSP 90 alpha ELISA Kit; Heat shock protein HSP 90 beta ELISA Kit; Heat shock protein HSP 90-alpha ELISA Kit; HS90A_HUMAN ELISA Kit; HSP 84 ELISA Kit; HSP 86 ELISA Kit; Hsp 90 ELISA Kit; HSP86 ELISA Kit; HSP90A ELISA Kit; HSP90AA1 ELISA Kit; HSP90AB1 ELISA Kit; HSP90B ELISA Kit; HSPC1 ELISA Kit; HSPC2 ELISA Kit; HSPCAL1 ELISA Kit; HSPCAL4 ELISA Kit; Renal carcinoma antigen NY-REN-38 ELISA Kit

缩写

HSP-90α

Uniprot No

P07900

灵敏度

0.195 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-90α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		93
		Range %		90-95
1:10		Average %		104
		Range %		100-107
1:20		Average %		94
		Range %		89-97
1:40		Average %		99
		Range %		92-103

回收率

The recovery of human HSP-90α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-97
EDTA plasma (n=4)		94		91-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 50 2.712 2.699 2.706 2.569 25 2.013 2.002 2.008 1.871 12.5 1.400 1.422 1.411 1.274 6.25 0.804 0.788 0.796 0.659 3.12 0.424 0.428 0.426 0.289 1.56 0.349 0.341 0.345 0.208 0.78 0.245 0.234 0.240 0.103 0 0.139 0.135 0.137

货期

3-5 working days