产品描述

This Human FMR1 ELISA Kit was designed for the quantitative measurement of Human FMR1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

FMR 1 ELISA Kit; Fmr1 ELISA Kit; Fmr1 gene ELISA Kit; FMR1_HUMAN ELISA Kit; FMRP ELISA Kit; Fragile X mental retardation 1 ELISA Kit; Fragile X mental retardation 1 protein ELISA Kit; Fragile X mental retardation protein 1 ELISA Kit; Fragile X mental retardation protein ELISA Kit; fragile X mental retardation syndrome-related protein 1 ELISA Kit; fragile X mental retardation; autosomal homolog 1 ELISA Kit; FRAXA ELISA Kit; fxr1 ELISA Kit; MGC87458 ELISA Kit; POF ELISA Kit; POF1 ELISA Kit; Protein FMR-1 ELISA Kit; Protein FMR1 ELISA Kit; wu:fb16f11 ELISA Kit; wu:fd18c10 ELISA Kit; zgc:66226 ELISA Kit

缩写

FMR1

Uniprot No

Q06787

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FMR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		85-93
1:2		Average %		107
		Range %		99-111
1:4		Average %		92
		Range %		85-95
1:8		Average %		99
		Range %		91-103

回收率

The recovery of human FMR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		85-95
EDTA plasma (n=4)		98		90-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1000 2.554 2.522 2.538 2.399   500 2.034 2.008 2.021 1.882   250 1.385 1.367 1.376 1.237   125 0.871 0.854 0.863 0.724   62.5 0.556 0.566 0.561 0.422   31.2 0.352 0.367 0.360 0.221   15.6 0.264 0.259 0.262 0.123   0 0.141 0.137 0.139

货期

3-5 working days