产品描述
IL-8是一种促炎性的细胞因子,也称为趋化因子,在细菌、病毒感染或损伤等情况下被活化的炎症细胞产生。它通过作用于白细胞表面的受体,在血管内皮细胞和周围组织引起炎症反应。IL-8在许多炎症性疾病中发挥作用,包括关节炎、哮喘和慢性阻塞性肺疾病等。因此,IL-8也成为了可能的治疗靶点。
华美生物所提供的Pig interleukin 8,IL-8 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测猪血清、血浆、细胞培养上清、组织匀浆样本中的IL8,其灵敏度为31.25 pg/mL,检测范围为125 pg/mL-8000 pg/mL。
别名
CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; Alveolar macrophage chemotactic factor I ELISA Kit; AMCF-I ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
缩写
CXCL8
Uniprot No
P26894
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:2 |
Average % |
90 |
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| Range % |
86-94 |
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| 1:4 |
Average % |
88 |
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| Range % |
84-93 |
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| 1:8 |
Average % |
92 |
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| Range % |
88-97 |
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回收率
| The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
95-104 |
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| EDTA plasma (n=4) |
85 |
81-90 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 8000 |
2.662 |
2.601 |
2.632 |
2.460 |
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| 4000 |
2.215 |
2.161 |
2.188 |
2.016 |
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| 2000 |
1.498 |
1.404 |
1.451 |
1.279 |
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| 1000 |
0.871 |
0.819 |
0.845 |
0.673 |
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| 500 |
0.510 |
0.507 |
0.509 |
0.337 |
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| 250 |
0.380 |
0.359 |
0.370 |
0.198 |
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| 125 |
0.271 |
0.261 |
0.266 |
0.094 |
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| 0 |
0.174 |
0.170 |
0.172 |
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货期
3-5 working days