产品描述

The human HIF-1α ELISA Kit allows for the in vitro quantitative determination of HIF-1α concentrations in serum, plasma, or tissue homogenates. This kit exclusively recognizes human HIF-1α. HIF-1α is a nuclear transcription factor that has been identified as a biomarker of the hypoxia microenvironment in most types of solid tumors. Under hypoxia, HIF-1α becomes relatively stable and binds to HIF-1β to form the active HIF-1 heterodimer. Many studies have reported that HIF-1α is detected in the majority of solid tumors including breast cancer, gastric cancer, and cervical cancer, and is linked to cell proliferation, metabolism, immune responses, apoptosis, genomic instability, vascularization, invasion, and metastasis of tumor cells. HIF-1α is also associated with chemo-/radioresistance. Pharmacological HIF-1α suppression is thus required for reversing chemo-/radioresistance in tumors.

The detection mechanism of this kit is based on the Sandwich-ELISA technique. HIF-1α in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled HIF-1α antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with HIF-1α content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.

别名

ARNT interacting protein ELISA Kit; ARNT-interacting protein ELISA Kit; Basic helix loop helix PAS protein MOP1 ELISA Kit; Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit; bHLHe78 ELISA Kit; Class E basic helix-loop-helix protein 78 ELISA Kit; HIF 1A ELISA Kit; HIF 1alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF-1alpha ELISA Kit; HIF-alpha ELISA Kit; HIF1 A ELISA Kit; HIF1 Alpha ELISA Kit; HIF1 ELISA Kit; HIF1-alpha ELISA Kit; HIF1A ELISA Kit; HIF1A_HUMAN ELISA Kit; hifla ELISA Kit; Hypoxia inducible factor 1 alpha ELISA Kit; Hypoxia inducible factor 1 alpha isoform I.3 ELISA Kit; Hypoxia inducible factor 1 alpha subunit ELISA Kit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor ELISA Kit; Hypoxia inducible factor 1; alpha subunit (basic helix loop helix transcription factor) ELISA Kit; Hypoxia inducible factor1alpha ELISA Kit; Hypoxia-inducible factor 1-alpha ELISA Kit; Member of PAS protein 1 ELISA Kit; Member of PAS superfamily 1 ELISA Kit; Member of the PAS Superfamily 1 ELISA Kit; MOP 1 ELISA Kit; MOP1 ELISA Kit; PAS domain-containing protein 8 ELISA Kit; PASD 8 ELISA Kit; PASD8 ELISA Kit

缩写

HIF-1α

Uniprot No

Q16665

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		84-96
1:2		Average %		96
		Range %		90-101
1:4		Average %		99
		Range %		95-103
1:8		Average %		94
		Range %		88-98

回收率

The recovery of human HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		91-98
EDTA plasma (n=4)		96		92-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 4000 2.737 2.718 2.728 2.619 2000 1.952 1.887 1.920 1.811 1000 1.342 1.331 1.337 1.228 500 0.776 0.767 0.772 0.663 250 0.407 0.399 0.403 0.294 125 0.309 0.302 0.306 0.197 62.5 0.195 0.188 0.192 0.083 0 0.111 0.107 0.109

货期

3-5 working days