产品描述

This Human FABP6 ELISA Kit was designed for the quantitative measurement of Human FABP6 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

Fabp6 ELISA Kit; FABP6_HUMAN ELISA Kit; Fatty acid binding protein 6; ileal (gastrotropin) ELISA Kit; Fatty acid-binding protein 6 ELISA Kit; Gastrotropin ELISA Kit; GT ELISA Kit; I 15P ELISA Kit; I BABP ELISA Kit; I BALB ELISA Kit; I BAP ELISA Kit; I-15P ELISA Kit; I-BABP ELISA Kit; I15P ELISA Kit; IBABP ELISA Kit; IBALB ELISA Kit; IBAP ELISA Kit; ILBP ELISA Kit; ILBP3 ELISA Kit; Ileal lipid binding protein ELISA Kit; Ileal lipid-binding protein ELISA Kit; ILLBP ELISA Kit; Intestinal 15 kDa protein ELISA Kit; Intestinal bile acid binding protein ELISA Kit; Intestinal bile acid-binding protein ELISA Kit

缩写

FABP6

Uniprot No

P51161

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human gastrotropin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		82-94
1:2		Average %		97
		Range %		91-101
1:4		Average %		94
		Range %		85-97
1:8		Average %		90
		Range %		84-95

回收率

The recovery of human gastrotropin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		91-104
EDTA plasma (n=4)		92		88-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 20 2.266 2.189 2.228 2.075 10 1.649 1.676 1.663 1.510 5 0.961 0.924 0.943 0.790 2.5 0.633 0.629 0.631 0.478 1.25 0.355 0.364 0.360 0.207 0.625 0.250 0.266 0.258 0.105 0.312 0.199 0.187 0.193 0.040 0 0.154 0.152 0.153

货期

3-5 working days