产品描述
This Pig IFN-β/IFNB ELISA Kit was designed for the quantitative measurement of Pig IFN-β/IFNB protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 50 pg/mL-2000 pg/mL and the sensitivity is 25 pg/mL.
缩写
IFN-β/IFNB
Uniprot No
Q29412
灵敏度
25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
85-86 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:4 |
Average % |
89 |
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| Range % |
84-93 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-99 |
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回收率
| The recovery of pig IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-101 |
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| EDTA plasma (n=4) |
84 |
80-88 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 2000 |
2.897 |
2.851 |
2.874 |
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| 1000 |
2.089 |
2.072 |
2.081 |
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| 500 |
1.196 |
1.163 |
1.180 |
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| 250 |
0.649 |
0.687 |
0.668 |
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| 100 |
0.286 |
0.293 |
0.290 |
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| 50 |
0.144 |
0.146 |
0.145 |
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货期
3-5 working days