产品描述

This Human DAG1 ELISA Kit was designed for the quantitative measurement of Human DAG1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

别名

156DAG ELISA Kit; A3a ELISA Kit; AGRNR ELISA Kit; Alpha dystroglycan ELISA Kit; Alpha-DG ELISA Kit; Beta-DG ELISA Kit; Beta-dystroglycan ELISA Kit; DAG ELISA Kit; Dag1 ELISA Kit; DAG1_HUMAN ELISA Kit; Dystroglycan 1 (dystrophin associated glycoprotein 1) ELISA Kit; Dystroglycan ELISA Kit; Dystrophin associated glycoprotein 1 ELISA Kit; Dystrophin-associated glycoprotein 1 ELISA Kit; OTTHUMP00000210857 ELISA Kit; OTTHUMP00000210858 ELISA Kit

缩写

DAG1

Uniprot No

Q14118

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human DAG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		86-98
1:2		Average %		98
		Range %		93-103
1:4		Average %		96
		Range %		91-102
1:8		Average %		95
		Range %		87-99

回收率

The recovery of human DAG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		88-97
EDTA plasma (n=4)		88		81-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 8000 2.301 2.159 2.230 2.098 4000 1.934 1.868 1.901 1.769 2000 1.562 1.534 1.548 1.416 1000 1.234 1.202 1.218 1.086 500 0.825 0.845 0.835 0.703 250 0.543 0.551 0.547 0.415 125 0.322 0.310 0.316 0.184 0 0.133 0.131 0.132

货期

3-5 working days