产品描述
This Pig IFNG ELISA Kit was designed for the quantitative measurement of Pig IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写
IFNG
Uniprot No
P17803
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-99 | |
| 1:2 | Average % | 92 | |
| Range % | 85-99 | |
| 1:4 | Average % | 95 | |
| Range % | 91-102 | |
| 1:8 | Average % | 95 | |
| Range % | 90-100 | |
回收率
| The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 86-102 | |
| EDTA plasma (n=4) | 96 | 89-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.692 | 2.647 | 2.670 | 2.581 | | | 500 | 2.404 | 2.465 | 2.435 | 2.346 | | | 250 | 1.974 | 1.989 | 1.982 | 1.893 | | | 125 | 1.523 | 1.541 | 1.532 | 1.443 | | | 62.5 | 0.945 | 0.942 | 0.944 | 0.855 | | | 31.2 | 0.613 | 0.635 | 0.624 | 0.535 | | | 15.6 | 0.337 | 0.324 | 0.331 | 0.242 | | | 0 | 0.090 | 0.087 | 0.089 | | |
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货期
3-5 working days