产品描述
This Mouse HEXA ELISA Kit was designed for the quantitative measurement of Mouse HEXA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 μU/mL-200 μU/mL and the sensitivity is 0.78 μU/mL.
别名
Hexa ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexosaminidase subunit A ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit
缩写
HEXA
Uniprot No
P29416
灵敏度
0.78 μU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:10 | Average % | 92 | |
| Range % | 85-100 | |
| 1:20 | Average % | 93 | |
| Range % | 87-98 | |
| 1:40 | Average % | 90 | |
| Range % | 86-100 | |
| 1:80 | Average % | 90 | |
| Range % | 86-98 | |
回收率
| The recovery of mouse β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 89-101 | |
| EDTA plasma (n=4) | 95 | 92-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| μU/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.542 | 2.517 | 2.530 | 2.386 | | | 100 | 2.073 | 2.101 | 2.087 | 1.943 | | | 50 | 1.452 | 1.464 | 1.458 | 1.314 | | | 25 | 0.942 | 0.961 | 0.952 | 0.808 | | | 12.5 | 0.416 | 0.402 | 0.409 | 0.265 | | | 6.25 | 0.325 | 0.311 | 0.318 | 0.174 | | | 3.12 | 0.247 | 0.237 | 0.242 | 0.098 | | | 0 | 0.146 | 0.141 | 0.144 | | |
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货期
3-5 working days