产品描述

This Human Hepatitis E virus antibody (IgG) ELISA Kit is designed to qualitatively detect the HEV IgG antibody in the serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HEV antigen. Samples or standards are pipetted into the wells with anti-human IgG conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human HEV IgG antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm.The valence of human HEV IgG antibody in the samples is determined by referring to the negative control. It indicates the presence of HEV IgG antibody if the O.D. (optical density) of sample is greater than or equal to the cutoff value (average Negative Control O.D. value plus 0.10). There is no HEV IgG antibody present in the sample if the O.D. is less than the cutoff value.

This assay has high sensitivity and excellent specificity for detection of human HEV IgG antibody. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.

HEV is a small, non-enveloped RNA virus that causes an acute, self-limited infection. Both anti-HEV IgM and IgG antibodies are produced after infection. Infected patients develop hepatitis symptoms following an incubation period of 15 to 60 days. Anti-HEV IgM antibodies appear in their serum at this time, followed by detectable anti-HEV IgG within a few days. HEV IgM antibody can still be detected 6 months after the onset of symptoms, while anti-HEV IgG usually persists for many years after infection. Anti-HEV IgG serological test can determine previous exposure to HEV. A positive result indicates prior or resolved HEV infection. A negative result indicates no past exposure to HEV.

缩写

灵敏度

反应时间

样本体积

检测波长

研究领域

测定原理

精密度

标准曲线

货期