产品描述
This Fish IFNG ELISA Kit was designed for the quantitative measurement of Fish IFNG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 20 pg/mL-4000 pg/mL and the sensitivity is 10 pg/mL.
别名
IFN-gamma,interferon gamma
缩写
IFN-
灵敏度
10 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 80-88 | |
| 1:2 | Average % | 92 | |
| Range % | 87-98 | |
| 1:4 | Average % | 100 | |
| Range % | 97-103 | |
| 1:8 | Average % | 53 | |
| Range % | 80-86 | |
回收率
| The recovery of fish IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 87-96 | |
| EDTA plasma (n=4) | 96 | 90-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | | | | 4000 | 0.155 | 0.158 | 0.157 | | | | 1000 | 0.311 | 0.321 | 0.316 | | | | 320 | 0.459 | 0.468 | 0.464 | | | | 80 | 0.861 | 0.842 | 0.852 | | | | 20 | 1.405 | 1.435 | 1.420 | | | | 0 | 2.458 | 2.311 | 2.385 | | |
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货期
3-5 working days