产品描述
This Rat Coagulation factor VII (F7) ELISA Kit was designed for the quantitative measurement of Rat Coagulation factor VII (F7) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.
别名
F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
缩写
F7
Uniprot No
Q8K3U6
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Blood Coagulation
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:200 | Average % | 91 | | |
| Range % | 86-98 | | |
| 1:400 | Average % | 97 | | |
| Range % | 92-106 | | |
| 1:800 | Average % | 93 | | |
| Range % | 88-99 | | |
| 1:1600 | Average % | 89 | | |
| Range % | 83-95 | | |
回收率
| The recovery of rat F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 87-97 | | |
| EDTA plasma (n=4) | 109 | 103-115 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 50 | 2.468 | 2.652 | 2.560 | 2.424 | | | | 25 | 2.115 | 2.076 | 2.096 | 1.960 | | | | 12.5 | 1.503 | 1.504 | 1.504 | 1.368 | | | | 6.25 | 0.845 | 0.805 | 0.825 | 0.689 | | | | 3.12 | 0.526 | 0.524 | 0.525 | 0.389 | | | | 1.56 | 0.381 | 0.359 | 0.370 | 0.234 | | | | 0.78 | 0.274 | 0.259 | 0.267 | 0.131 | | | | 0 | 0.137 | 0.135 | 0.136 | | | |
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货期
3-5 working days