产品描述
This Rat F-TESTO ELISA Kit was designed for the quantitative measurement of Rat F-TESTO protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.3 pg/mL-60 pg/mL and the sensitivity is 0.15 pg/mL.
缩写
F-TESTO
灵敏度
0.15 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:2 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of rat F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
89-104 |
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| EDTA plasma (n=4) |
94 |
88-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 60 |
0.202 |
0.205 |
0.204 |
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| 15 |
0.359 |
0.342 |
0.351 |
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| 4.8 |
0.610 |
0.623 |
0.617 |
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| 1.2 |
1.007 |
1.012 |
1.010 |
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| 0.3 |
1.464 |
1.457 |
1.461 |
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| 0 |
2.439 |
2.454 |
2.447 |
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货期
3-5 working days