产品描述
The mouse DLK1 ELISA Kit is used to quantitatively measure mouse DLK1 levels in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of DLK1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
DLK1, a noncanonical ligand of notch signaling, plays an essential in normal developmental processes, including neuroendocrine differentiation, hepatocyte and biliary epithelial cell differentiation, hematopoiesis, osteogenesis, skeletal muscle differentiation, and chondrogenic differentiation. DLK1 is involved in the regulation of stem cell pools, tissue differentiation during development, cancer differentiation, and cancer stem-like cell (CSCs) maintenance. It has been detected in various carcinomas including neuroendocrine tumors.
别名
Dlk1 ELISA kit; Dlk ELISA kit; Pref1 ELISA kit; Scp-1Protein delta homolog 1 ELISA kit; DLK-1 ELISA kit; Adipocyte differentiation inhibitor protein ELISA kit; Preadipocyte factor 1 ELISA kit; Pref-1) [Cleaved into: Fetal antigen 1 ELISA kit; FA1)] ELISA kit
缩写
DLK1
Uniprot No
Q09163
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse DLK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 84-96 | |
| 1:2 | Average % | 95 | |
| Range % | 91-99 | |
| 1:4 | Average % | 94 | |
| Range % | 90-98 | |
| 1:8 | Average % | 102 | |
| Range % | 92-108 | |
回收率
| The recovery of mouse DLK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 92-102 | |
| EDTA plasma (n=4) | 89 | 82-98 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.045 | 1.944 | 1.995 | 1.844 | | | 500 | 1.644 | 1.523 | 1.584 | 1.433 | | | 250 | 1.073 | 1.052 | 1.063 | 0.912 | | | 125 | 0.673 | 0.662 | 0.668 | 0.517 | | | 62.5 | 0.371 | 0.382 | 0.377 | 0.226 | | | 31.2 | 0.284 | 0.273 | 0.279 | 0.128 | | | 15.6 | 0.235 | 0.231 | 0.233 | 0.082 | | | 0 | 0.148 | 0.153 | 0.151 | | |
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
