产品描述
This Rabbit IFNG ELISA Kit was designed for the quantitative measurement of Rabbit IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
别名
IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写
IFNG
Uniprot No
P30123
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 85-105 | |
| 1:2 | Average % | 94 | |
| Range % | 85-105 | |
| 1:4 | Average % | 96 | |
| Range % | 92-100 | |
| 1:8 | Average % | 95 | |
| Range % | 83-105 | |
回收率
| The recovery of rabbit IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 85-100 | |
| EDTA plasma (n=4) | 95 | 85-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 2.862 | 2.713 | 2.788 | 2.673 | | | 2000 | 2.214 | 2.229 | 2.222 | 2.107 | | | 1000 | 1.373 | 1.397 | 1.385 | 1.270 | | | 500 | 0.755 | 0.731 | 0.743 | 0.628 | | | 250 | 0.446 | 0.432 | 0.439 | 0.324 | | | 125 | 0.263 | 0.275 | 0.269 | 0.154 | | | 62.5 | 0.202 | 0.194 | 0.198 | 0.083 | | | 0 | 0.117 | 0.113 | 0.115 | | |
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货期
3-5 working days