产品描述
The product CSB-E13027r is a sandwich ELISA kit developed to measure concentrations of rat FABP4 in serum, plasma, cell lysates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of FABP4 in samples. The color intensity is measured at 450 nm via a microplate reader.
FABP4, also known as A-FABP or aP2, is mainly expressed in adipocytes and functions as a lipid chaperone protein. It regulates lipolysis and adipogenesis through interactions with hormone-sensitive lipase (HSL) and peroxisome PPAR-γ, respectively. FABP4 plays an important role in the development of insulin resistance and atherosclerosis in association with metaflammation. An increase in circulating FABP4 concentrations is linked to obesity, insulin resistance, diabetes, hypertension, cardiac dysfunction, and atherosclerosis. Reducing FABP4 levels or activity is associated with improved metabolic health.
别名
Fabp4Fatty acid-binding protein ELISA Kit; adipocyte ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; ALBP ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; A-FABP ELISA Kit; AFABP ELISA Kit; Fatty acid-binding protein 4 ELISA Kit
缩写
FABP4
Uniprot No
P70623
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
88-100 |
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| 1:2 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:4 |
Average % |
89 |
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| Range % |
84-99 |
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| 1:8 |
Average % |
92 |
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| Range % |
86-101 |
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回收率
| The recovery of rat FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
90 |
82-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
1.974 |
1.913 |
1.944 |
1.817 |
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| 50 |
1.721 |
1.681 |
1.701 |
1.574 |
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| 25 |
1.416 |
1.311 |
1.364 |
1.237 |
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| 12.5 |
0.958 |
0.897 |
0.928 |
0.801 |
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| 6.25 |
0.669 |
0.629 |
0.649 |
0.522 |
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| 3.12 |
0.395 |
0.364 |
0.380 |
0.253 |
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| 1.56 |
0.217 |
0.207 |
0.212 |
0.085 |
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| 0 |
0.131 |
0.123 |
0.127 |
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货期
3-5 working days
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