产品描述

This Human ID1 ELISA Kit was designed for the quantitative measurement of Human ID1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

别名

bHLHb24 ELISA Kit; Class B basic helix-loop-helix protein 24 ELISA Kit; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) ELISA Kit; DNA binding protein inhibitor ID 1 ELISA Kit; DNA binding protein inhibitor ID1 ELISA Kit; DNA-binding protein inhibitor ID-1 ELISA Kit; Dominant negative helix loop helix protein ELISA Kit; ID 1 ELISA Kit; ID ELISA Kit; ID1 ELISA Kit; ID1_HUMAN ELISA Kit; Inhibitor of Differentiation 1 ELISA Kit; Inhibitor of DNA binding 1 ELISA Kit; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein ELISA Kit

缩写

ID1

Uniprot No

P41134

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ID1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		82-93
1:2		Average %		98
		Range %		94-100
1:4		Average %		97
		Range %		90-104
1:8		Average %		85
		Range %		82-93

回收率

The recovery of human ID1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-95
EDTA plasma (n=4)		101		96-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.654 2.647 2.651 2.501 1000 2.222 2.327 2.275 2.125 500 1.567 1.544 1.556 1.406 250 0.785 0.745 0.765 0.615 125 0.437 0.433 0.435 0.285 62.5 0.358 0.347 0.353 0.203 31.25 0.246 0.254 0.250 0.100 0 0.151 0.148 0.150

货期

3-5 working days