产品描述

This Human FGF8 ELISA Kit was designed for the quantitative measurement of Human FGF8 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

AIGF ELISA Kit; Androgen induced growth factor ELISA Kit; Androgen-induced growth factor ELISA Kit; FGF 8 ELISA Kit; FGF-8 ELISA Kit; FGF-8b ELISA Kit; FGF8 ELISA Kit; FGF8_HUMAN ELISA Kit; Fibroblast growth factor 8 (androgen induced) ELISA Kit; Fibroblast growth factor 8 ELISA Kit; Fibroblast growth factor 8 precursor ELISA Kit; HBGF 8 ELISA Kit; HBGF-8 ELISA Kit; HBGF8 ELISA Kit; Heparin-binding growth factor 8 ELISA Kit; HH6 ELISA Kit; KAL6 ELISA Kit

缩写

FGF8

Uniprot No

P55075

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-95
1:2		Average %		102
		Range %		97-107
1:4		Average %		91
		Range %		85-97
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human FGF8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.455 2.498 2.477 2.290 500 1.872 1.932 1.902 1.715 250 1.213 1.236 1.225 1.038 125 0.687 0.699 0.693 0.506 62.5 0.499 0.484 0.492 0.305 31.2 0.379 0.398 0.389 0.202 15.6 0.277 0.287 0.282 0.095 0 0.188 0.186 0.187

货期

3-5 working days