产品描述
This Human HSP72 ELISA Kit was designed for the quantitative measurement of Human HSP72 protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
别名
HSPA1A ELISA Kit; HSP72 ELISA Kit; HSPA1 ELISA Kit; HSX70Heat shock 70 kDa protein 1A ELISA Kit; Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit; HSP70.1 ELISA Kit
缩写
HSP-72
Uniprot No
P0DMV8
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
84 |
|
| Range % |
80-90 |
|
| 1:2 |
Average % |
98 |
|
| Range % |
91-103 |
|
| 1:4 |
Average % |
101 |
|
| Range % |
92-108 |
|
| 1:8 |
Average % |
93 |
|
| Range % |
86-96 |
|
回收率
| The recovery of human HSP-72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-98 |
|
| EDTA plasma (n=4) |
99 |
92-107 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 200 |
2.868 |
2.856 |
2.862 |
2.706 |
|
| 100 |
2.436 |
2.387 |
2.412 |
2.256 |
|
| 50 |
1.694 |
1.668 |
1.681 |
1.525 |
|
| 25 |
1.034 |
1.024 |
1.029 |
0.873 |
|
| 12.5 |
0.594 |
0.587 |
0.591 |
0.435 |
|
| 6.25 |
0.415 |
0.402 |
0.409 |
0.253 |
|
| 3.12 |
0.283 |
0.273 |
0.278 |
0.122 |
|
| 0 |
0.158 |
0.154 |
0.156 |
|
|
|
货期
3-5 working days