产品描述

This Human EGFL6 ELISA Kit was designed for the quantitative measurement of Human EGFL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

别名

EGF like domain multiple 6 ELISA Kit; EGF repeat containing protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; EGF-like-domain multiple 6 ELISA Kit; egfl6 ELISA Kit; EGFL6_HUMAN ELISA Kit; epidermal growth factor like protein 6 ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; MAEG ELISA Kit; MAM and EGF domain containing ELISA Kit; MAM and EGF domains containing gene protein ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; W80 ELISA Kit

缩写

EGFL6

Uniprot No

Q8IUX8

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		104
		Range %		97-108
1:2		Average %		101
		Range %		93-105
1:4		Average %		97
		Range %		89-101
1:8		Average %		104
		Range %		94-109

回收率

The recovery of human EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		83-94
EDTA plasma (n=4)		102		95-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 5000 1.972 1.918 1.945 1.800 2500 1.400 1.439 1.420 1.275 1250 1.072 1.025 1.049 0.904 625 0.762 0.734 0.748 0.603 312 0.521 0.529 0.525 0.380 156 0.348 0.357 0.353 0.208 78 0.271 0.283 0.277 0.132 0 0.148 0.142 0.145

货期

3-5 working days