产品描述
This Human GR-β ELISA Kit was designed for the quantitative measurement of Human GR-β protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.
别名
lucocorticoid receptor-β,GR-β|lucocorticoid receptor-beta,GR-beta
缩写
GR-β
灵敏度
9.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays):CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GR-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
103 |
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| Range % |
99-108 |
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| 1:2 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:4 |
Average % |
91 |
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| Range % |
88-95 |
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| 1:8 |
Average % |
89 |
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| Range % |
85-96 |
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回收率
| The recovery of human GR-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
87 |
83-95 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2500 |
2.441 |
2.382 |
2.412 |
2.301 |
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| 1250 |
1.692 |
1.725 |
1.709 |
1.598 |
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| 625 |
1.021 |
1.103 |
1.062 |
0.951 |
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| 312.5 |
0.563 |
0.539 |
0.551 |
0.440 |
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| 156 |
0.327 |
0.314 |
0.321 |
0.210 |
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| 78 |
0.245 |
0.258 |
0.252 |
0.141 |
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| 39 |
0.185 |
0.191 |
0.188 |
0.077 |
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| 0 |
0.108 |
0.113 |
0.111 |
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货期
3-5 working days