产品描述
This Rabbit IGF1 ELISA Kit was designed for the quantitative measurement of Rabbit IGF1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
别名
IGF1 ELISA Kit; IGF-1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写
IGF1
Uniprot No
Q95222
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 80-100 | |
| 1:2 | Average % | 100 | |
| Range % | 91-105 | |
| 1:4 | Average % | 98 | |
| Range % | 92-115 | |
| 1:8 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of rabbit IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 90 | 85-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.715 | 2.725 | 2.720 | 2.605 | | | 5 | 2.035 | 2.002 | 2.019 | 1.904 | | | 2.5 | 0.989 | 1.078 | 1.034 | 0.919 | | | 1.25 | 0.591 | 0.602 | 0.597 | 0.482 | | | 0.625 | 0.342 | 0.352 | 0.347 | 0.232 | | | 0.312 | 0.201 | 0.212 | 0.207 | 0.092 | | | 0.156 | 0.175 | 0.185 | 0.180 | 0.065 | | | 0 | 0.111 | 0.119 | 0.115 | | |
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货期
3-5 working days