产品描述
This Rat EDN1 ELISA Kit was designed for the quantitative measurement of Rat EDN1 protein in serum, plasma, cell lysates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 pg/mL-80 pg/mL and the sensitivity is 0.31 pg/mL .
别名
Edn1 ELISA Kit; Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
缩写
EDN1
Uniprot No
P22388
灵敏度
0.31 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 84-91 | |
| 1:2 | Average % | 96 | |
| Range % | 91-102 | |
| 1:4 | Average % | 84 | |
| Range % | 81-87 | |
| 1:8 | Average % | 92 | |
| Range % | 88-96 | |
回收率
| The recovery of rat ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-96 | |
| EDTA plasma (n=4) | 95 | 90-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 80 | 2.085 | 2.086 | 2.086 | 1.951 | | | 40 | 1.540 | 1.649 | 1.595 | 1.460 | | | 20 | 1.111 | 1.156 | 1.134 | 0.999 | | | 10 | 0.718 | 0.750 | 0.734 | 0.599 | | | 5 | 0.407 | 0.405 | 0.406 | 0.271 | | | 2.5 | 0.231 | 0.237 | 0.234 | 0.099 | | | 1.25 | 0.181 | 0.173 | 0.177 | 0.042 | | | 0 | 0.129 | 0.140 | 0.135 | | |
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货期
3-5 working days