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产品描述
The Human hepatitis D virus (HDV) IgG ELISA kit is used for qualitative identification of IgG antibodies to HDV in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HDV antigen. Samples or standards are pipetted into the wells with anti-human IgG conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human HDV IgG antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. The valence of human HDV IgG antibody in the samples is determined by referring to the negative control. It indicates the presence of HDV IgG antibody if the O.D. (optical density) of sample is greater than or equal to the cutoff value (average Negative Control value plus 0.10). There is no HDV IgG antibody present in the sample if the O.D. is less than the cutoff value.
This assay has high sensitivity and excellent specificity for detection of human HDV IgG antibody. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.
HDV is a defective RNA virus and is comprised of a delta antigen (viral core) and a hepatitis B surface antigen (HBsAg) (viral protein coat). HBV is required to initiate and maintain the replication of HDV in the infected liver cells. HDV is also known as a "satellite virus" because HDV-infected patients are usually infected with HBV. The diagnosis of HDV can be done by detecting the HDV antigen, HDV-specific IgM, or HDV-specific total antibodies (IgM and IgG) in the serum of patients with clinically obvious acute or chronic hepatitis B infection. Anti-HDV IgM usually appears in serum at 2 to 3 weeks after onset of symptoms and disappears by 2 months after acute HDV infection. IgM antibodies may persist for 9 months in HDV reinfection. HDV IgG and HDV total antibodies last in serum after the resolution of acute HDV infection and in chronic coinfection. IgG antibodies usually appear in the recovery period after a few weeks.
别名
N/A缩写
HDV Ab (IgG)灵敏度
Request Information反应时间
1-5h样本体积
50-100ul检测波长
450 nm研究领域
Microbiology精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||
标准曲线
| Test parameter | specification | test result | |||||||
| Positive control | >0.6 | 0.842 | |||||||
| Negative control | <0.1 | 0.051 | |||||||
| Positive rate | 10,Positive | 100% | |||||||
| Negative rate | 10,Negative | 100% | |||||||
货期
3-5 working days
