产品描述
This Sheep IFNG ELISA Kit was designed for the quantitative measurement of Sheep IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 5 pg/mL-200 pg/mL and the sensitivity is 2.5 pg/mL.
别名
IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写
IFNG
Uniprot No
P17773
灵敏度
2.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
95-102 |
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| 1:2 |
Average % |
87 |
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| Range % |
84-90 |
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| 1:4 |
Average % |
85 |
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| Range % |
82-89 |
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| 1:8 |
Average % |
93 |
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| Range % |
90-96 |
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回收率
| The recovery of sheep IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-95 |
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| EDTA plasma (n=4) |
86 |
83-89 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
2.776 |
2.798 |
2.787 |
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| 100 |
2.088 |
2.098 |
2.093 |
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| 50 |
1.164 |
1.187 |
1.176 |
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| 25 |
0.675 |
0.633 |
0.654 |
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| 10 |
0.264 |
0.287 |
0.276 |
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| 5 |
0.125 |
0.127 |
0.126 |
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货期
3-5 working days