产品描述

The human VEGFD ELISA Kit is intended to measure human VEGFD in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human VEGFD concentration. The intensity of the colored product is directly proportional to the concentration of VEGFD present in the sample. This human VEGFD ELISA kit has high specificity and excellent sensitivity.

VEGFD is a secreted glycoprotein that can activate VEGFR-2 and VEGFR-3 on the endothelium and acts as a mitogen for endothelial cells to promote the growth and remodeling of blood vessels and lymphatic vessels. VEGFD is a potential biomarker for lymphangioleiomyomatosis. It has been demonstrated that high serum levels of VEGFD are linked to atrial fibrillation, ischemic stroke, and heart failure. Elevated VEGFD values have recently been associated with all-cause mortality in individuals with coronary artery disease.

别名

c-fos induced growth factor ELISA Kit; c-fos induced growth factor (vascular endothelial growth factor D) ELISA Kit; c-fos induced growth factor ELISA Kit; c-fos-induced growth factor ELISA Kit; FIGF ELISA Kit; Vascular endothelial growth factor D ELISA Kit; Vascular endothelial growth factor D precursor ELISA Kit; Vascular endothelial growth factor D precursor ELISA Kit; VEGF D ELISA Kit; VEGF-D ELISA Kit; VEGFD ELISA Kit; VEGFD_HUMAN ELISA Kit

缩写

FIGF

Uniprot No

O43915

灵敏度

7.81 pg/ml

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
Range %	88-98
1:2	Average %	102
Range %	92-106
1:4	Average %	98
Range %	89-102
1:8	Average %	94
Range %	87-98

回收率

The recovery of human VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		84-96
EDTA plasma (n=4)		99		91-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   4000 2.436 2.568 2.502 2.352   2000 2.062 2.132 2.097 1.947   1000 1.457 1.444 1.451 1.301   500 0.760 0.789 0.775 0.625   250 0.486 0.458 0.472 0.322   125 0.372 0.360 0.366 0.216   62.5 0.255 0.247 0.251 0.101   0 0.152 0.148 0.150

货期

3-5 working days