产品描述
The rat heart fatty acid-binding protein (h-FABP) ELISA Kit is used to quantitatively measure rat h-FABP levels in serum, plasma, cell culture supernates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of h-FABP in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
h-FABP, also called FABP3, is ubiquitously expressed in the heart, skeletal muscle, and other tissues. It participates in maintaining an energy supply to the heart and other body parts, as well as in regulating the intramuscular fat content and improving insulin sensitivity. FABP3 binds to free long-chain fatty acids (LCFAs) and transports them for cell metabolism, thus protecting against lipid toxicity. Studies have demonstrated that deficiencies in FABP3 exacerbate metabolic derangement in cardiac Hypertrophy and heart failure via the PPARα pathway.
别名
Fabp3Fatty acid-binding protein ELISA kit; heart ELISA kit; Fatty acid-binding protein 3 ELISA kit; Heart-type fatty acid-binding protein ELISA kit; H-FABP ELISA kit
缩写
FABP3
Uniprot No
P07483
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat h-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 84-92 | |
| 1:2 | Average % | 96 | |
| Range % | 91-102 | |
| 1:4 | Average % | 84 | |
| Range % | 81-88 | |
| 1:8 | Average % | 94 | |
| Range % | 90-98 | |
回收率
| The recovery of rat h-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-95 | |
| EDTA plasma (n=4) | 103 | 95-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.095 | 2.086 | 2.091 | 1.967 | | | 500 | 1.550 | 1.648 | 1.599 | 1.475 | | | 250 | 1.121 | 1.156 | 1.139 | 1.015 | | | 125 | 0.728 | 0.751 | 0.740 | 0.616 | | | 62.5 | 0.417 | 0.403 | 0.410 | 0.286 | | | 31.2 | 0.241 | 0.237 | 0.239 | 0.115 | | | 15.6 | 0.211 | 0.214 | 0.213 | 0.089 | | | 0 | 0.119 | 0.129 | 0.124 | | |
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货期
3-5 working days
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