产品描述
This Rat HMOX1 ELISA Kit was designed for the quantitative measurement of Rat HMOX1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Hmox1Heme oxygenase 1 ELISA Kit; HO-1 ELISA Kit; EC 1.14.14.18 ELISA Kit; HSP32 ELISA Kit
缩写
HMOX1
Uniprot No
P06762
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 94-102 | |
| 1:2 | Average % | 100 | |
| Range % | 96-107 | |
| 1:4 | Average % | 86 | |
| Range % | 82-90 | |
| 1:8 | Average % | 94 | |
| Range % | 90-100 | |
回收率
| The recovery of rat HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 87-99 | |
| EDTA plasma (n=4) | 96 | 91-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.323 | 2.252 | 2.288 | 2.185 | | | 10 | 1.776 | 1.692 | 1.734 | 1.631 | | | 5 | 1.182 | 1.105 | 1.144 | 1.041 | | | 2.5 | 0.725 | 0.743 | 0.734 | 0.631 | | | 1.25 | 0.465 | 0.443 | 0.454 | 0.351 | | | 0.625 | 0.275 | 0.259 | 0.267 | 0.164 | | | 0.312 | 0.192 | 0.207 | 0.200 | 0.097 | | | 0 | 0.104 | 0.102 | 0.103 | | |
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货期
3-5 working days