产品描述

This Human HBEGF ELISA Kit was designed for the quantitative measurement of Human HBEGF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

别名

diphtheria toxin receptor (heparin-binding EGF-like growth factor) ELISA Kit; diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) ELISA Kit; Diphtheria toxin receptor ELISA Kit; DT R ELISA Kit; DT-R ELISA Kit; DTR ELISA Kit; DTS ELISA Kit; DTSF ELISA Kit; HB-EGF ELISA Kit; HBEGF ELISA Kit; HBEGF_HUMAN ELISA Kit; HEGFL ELISA Kit; Heparin binding EGF like growth factor ELISA Kit; Heparin binding epidermal growth factor ELISA Kit; Heparin binding epidermal growth factor like growth factor ELISA Kit; Heparin-binding EGF-like growth factor ELISA Kit; Proheparin binding EGF like growth factor ELISA Kit

缩写

HBEGF

Uniprot No

Q99075

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HB-EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		84-93
1:2		Average %		86
		Range %		82-91
1:4		Average %		92
		Range %		85-96
1:8		Average %		105
		Range %		98-109

回收率

The recovery of human HB-EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		88		83-92
EDTA plasma (n=4)		97		92-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     4000 2.214 2.267 2.241 2.141     2000 1.291 1.391 1.341 1.241     1000 0.699 0.745 0.722 0.622     500 0.418 0.412 0.415 0.315     250 0.277 0.284 0.281 0.181     125 0.169 0.175 0.172 0.072     62.5 0.135 0.136 0.136 0.036     0 0.099 0.100 0.100

货期

3-5 working days