产品描述
This Mouse HGFAC ELISA Kit was designed for the quantitative measurement of Mouse HGFAC protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
Hgfac ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
缩写
HGFAC
Uniprot No
Q9R098
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HGFAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 104 | | |
| Range % | 98-109 | | |
| 1:2 | Average % | 97 | | |
| Range % | 90-101 | | |
| 1:4 | Average % | 92 | | |
| Range % | 86-95 | | |
| 1:8 | Average % | 97 | | |
| Range % | 93-102 | | |
回收率
| The recovery of mouse HGFAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 94 | 87-98 | | |
| EDTA plasma (n=4) | 98 | 92-103 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 2000 | 2.580 | 2.469 | 2.525 | 2.355 | | | | 1000 | 1.826 | 1.902 | 1.864 | 1.694 | | | | 500 | 1.203 | 1.183 | 1.193 | 1.023 | | | | 250 | 0.682 | 0.664 | 0.673 | 0.503 | | | | 125 | 0.399 | 0.385 | 0.392 | 0.222 | | | | 62.5 | 0.277 | 0.284 | 0.281 | 0.111 | | | | 31.25 | 0.203 | 0.209 | 0.206 | 0.036 | | | | 0 | 0.169 | 0.171 | 0.170 | | | |
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货期
3-5 working days