产品描述

This Human EBI3 ELISA Kit was designed for the quantitative measurement of Human EBI3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

cytokine receptor ELISA Kit; EBI3 ELISA Kit; EBV induced gene 3 protein ELISA Kit; EBV-induced gene 3 protein ELISA Kit; Epstein Barr virus induced 3 ELISA Kit; Epstein Barr virus induced gene 3 ELISA Kit; Epstein Barr virus induced gene 3 protein ELISA Kit; Epstein-Barr virus-induced gene 3 protein ELISA Kit; IL 27 subunit beta ELISA Kit; IL 27B ELISA Kit; IL-27 subunit beta ELISA Kit; IL-27B ELISA Kit; IL27 subunit ELISA Kit; IL27 subunit beta ELISA Kit; IL27B ELISA Kit; IL27B_HUMAN ELISA Kit; IL35 subunit ELISA Kit; interleukin 27 subunit beta ELISA Kit; Interleukin-27 subunit beta ELISA Kit

缩写

EBI3

Uniprot No

Q14213

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human EBI3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		91-100
1:2		Average %		102
		Range %		98-105
1:4		Average %		86
		Range %		82-90
1:8		Average %		93
		Range %		88-96

回收率

The recovery of human EBI3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		84-92
EDTA plasma (n=4)		101		96-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.643 2.589 2.616 2.476 500 1.678 1.645 1.662 1.522 250 1.056 1.033 1.045 0.905 125 0.702 0.714 0.708 0.568 62.5 0.452 0.476 0.464 0.324 31.25 0.297 0.308 0.303 0.163 15.6 0.215 0.222 0.219 0.079 0 0.139 0.141 0.140

货期

3-5 working days