产品描述

This Human DNASE1 ELISA Kit was designed for the quantitative measurement of Human DNASE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

别名

Deoxyribonuclease 1 ELISA Kit; Deoxyribonuclease I ELISA Kit; Deoxyribonuclease-1 ELISA Kit; Deoxyribonuclease1 ELISA Kit; DeoxyribonucleaseI ELISA Kit; DNAS1_HUMAN ELISA Kit; DNASE 1 ELISA Kit; DNase I ELISA Kit; DNase I lysosomal ELISA Kit; DNASE1 ELISA Kit; DNaseI ELISA Kit; DNL 1 ELISA Kit; DNL1 ELISA Kit; Dornase alfa ELISA Kit; DRNI ELISA Kit; FLJ38093 ELISA Kit; Human urine deoxyribonuclease I ELISA Kit

缩写

DNASE1

Uniprot No

P24855

灵敏度

0.39 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human DNase-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		83-92
1:2		Average %		110
		Range %		105-115
1:4		Average %		85
		Range %		81-90
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human DNase-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		87		85-90
EDTA plasma (n=4)		95		89-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 100 2.562 2.598 2.580 2.435 50 2.073 2.098 2.086 1.941 25 1.314 1.365 1.340 1.195 12.5 0.695 0.668 0.682 0.537 6.25 0.432 0.451 0.442 0.297 3.12 0.287 0.268 0.278 0.133 1.56 0.204 0.209 0.207 0.062 0 0.143 0.146 0.145

货期

3-5 working days