产品描述
This Fish GSH-PX ELISA Kit was designed for the quantitative measurement of Fish GSH-PX protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 375 μU/mL-6000 μU/mL and the sensitivity is 187.5 μU/mL.
缩写
GSH-PX
灵敏度
187.5 μU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:200 |
Average % |
103 |
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| Range % |
99-107 |
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| 1:400 |
Average % |
98 |
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| Range % |
93-103 |
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| 1:800 |
Average % |
92 |
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| Range % |
87-97 |
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回收率
| The recovery of fish GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
94-104 |
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| EDTA plasma (n=4) |
89 |
84-94 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μU/ml |
OD1 |
OD2 |
Average |
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| 6000 |
0.089 |
0.084 |
0.087 |
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| 3000 |
0.246 |
0.265 |
0.256 |
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| 1500 |
0.551 |
0.580 |
0.566 |
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| 750 |
0.933 |
0.981 |
0.957 |
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| 375 |
1.646 |
1.569 |
1.608 |
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| 0 |
2.362 |
2.398 |
2.380 |
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货期
3-5 working days