产品描述

This Human FOXO3 ELISA Kit was designed for the quantitative measurement of Human FOXO3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

别名

AF6q21 ELISA Kit; AF6q21 protein ELISA Kit; DKFZp781A0677 ELISA Kit; FKHR2 ELISA Kit; FKHRL 1 ELISA Kit; FKHRL1 ELISA Kit; FKHRL1P2 ELISA Kit; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 ELISA Kit; Forkhead box O3 ELISA Kit; Forkhead box O3A ELISA Kit; Forkhead box protein O3 ELISA Kit; Forkhead box protein O3A ELISA Kit; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 ELISA Kit; Forkhead homolog (rhabdomyosarcoma) like 1 ELISA Kit; Forkhead in rhabdomyosarcoma like 1 ELISA Kit; Forkhead in rhabdomyosarcoma-like 1 ELISA Kit; FOX O3A ELISA Kit; FOXO2 ELISA Kit; foxo3 ELISA Kit; FOXO3_HUMAN ELISA Kit; FOXO3A ELISA Kit; MGC12739 ELISA Kit; MGC31925 ELISA Kit

缩写

FOXO3

Uniprot No

O43524

灵敏度

0.39 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FOXO3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		87
		Range %		80-92
1:200		Average %		99
		Range %		91-105
1:400		Average %		101
		Range %		92-110
1:800		Average %		93
		Range %		86-98

回收率

The recovery of human FOXO3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-98
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 100 2.943 2.903 2.923 2.818 50 2.417 2.348 2.383 2.278 25 1.685 1.663 1.674 1.569 12.5 1.075 0.989 1.032 0.927 6.25 0.597 0.603 0.600 0.495 3.12 0.374 0.357 0.366 0.261 1.56 0.236 0.229 0.233 0.128 0 0.109 0.101 0.105

货期

3-5 working days