产品描述

This Mouse ENPP1 ELISA Kit was designed for the quantitative measurement of Mouse ENPP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

Enpp1 ELISA kit; Npps ELISA kit; Pc1 ELISA kit; Pdnp1Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA kit; E-NPP 1 ELISA kit; Lymphocyte antigen 41 ELISA kit; Ly-41 ELISA kit; Phosphodiesterase I/nucleotide pyrophosphatase 1 ELISA kit; Plasma-cell membrane glycoprotein PC-1) [Cleaved into: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA kit; secreted form] [Includes: Alkaline phosphodiesterase I ELISA kit; EC 3.1.4.1); Nucleotide pyrophosphatase ELISA kit; NPPase ELISA kit; EC 3.6.1.9 ELISA kit; Nucleotide diphosphatase)] ELISA kit

缩写

ENPP1

Uniprot No

P06802

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		93
		Range %		89-97
1:10		Average %		90
		Range %		82-99
1:20		Average %		95
		Range %		87-101
1:40		Average %		95
		Range %		85-104

回收率

The recovery of mouse ENPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		88-99
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 20 2.591 2.575 2.583 2.489 10 1.465 1.492 1.479 1.385 5 0.786 0.802 0.794 0.700 2.5 0.424 0.438 0.431 0.337 1.25 0.272 0.274 0.273 0.179 0.625 0.168 0.172 0.170 0.076 0.312 0.127 0.129 0.128 0.034 0 0.092 0.095 0.094

货期

3-5 working days